Biochemistry In The Lab: A Manual For Undergraduates

Description

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.

Key Features

• Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience
• Includes chemical biology as its foundation, teaching readers experimental methods specific to the field
• Provides instructor experiments that are easy to prepare and execute, at comparatively low cost
• Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry
• Is written by an expert in the field

Table of Contents

1. Buffers
2. Assays
3. ELISA
4. Protein Concentration
5. Salting out Proteins and other Biomolecules
6. A Discussion of Isoelectric Point and Effective Charge
7. Column Chromatography
8. Michaelis-Menten Kinetics
9. Protein Purification
10. Polyacrylamide Gels
11. in silico Biochemistry: the evolution of globins
12. Growing Crystals of Hemoglobin
13. Enzyme Inhibition
14. Multisubstrate Kinetics
15. Fluorescence and Denaturation
16. Fluorescence Studies of Ligand Binding
17. DNA Restriction Digests
18. Western Blotting

Author Biography

Dr. Benjamin F. Lasseter earned his Ph.D. in Biochemistry from Texas A&M University in 2003. He briefly assisted in the production of history textbooks for the Catholic Schools Textbook Project before teaching at a high school in Louisiana. He moved on to Christopher Newport University in 2011, where he teaches the biochemistry labs. He mixes analytical chemistry with pure biology to study environmental impacts of metal pollution.