Developmental Toxicology: Methods and Protocols (2nd Edition)

Description

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab.

Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures.

Table of Contents

1. In Vitro Models in Developmental Toxicology – Louise M. Winn
2. Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments – Jason M. Hansen, Ted B. Piorczynski
3. Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology – Charles A. Easley
4. Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model – Mika Suga, Miho K. Furue
5. Micromass Methods for the Evaluation of Developmental Toxicants – Omar J. Mohammed, Margaret K. Pratten
6. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System – France-Hélène Paradis, Han Yan, Chunwei Huang, Barbara F. Hales
7. Embryonic Midfacial Palatal Organ Culture Methods in Developmental Toxicology – Barbara D. Abbott
8. Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology – Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, Sakhila K. Banu
9. Hepatotoxicity in Zebrafish Larvae – Kathryn Bambino, Joshua Morrison, Jaime Chu
10. Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model – Joslynn G. Affleck, Virginia K. Walker
11. The Chicken Embryo as a Model in Developmental Toxicology – Michael R. Stark, Micah M. Ross
12. The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity – Cecilia Berg
13. Mouse Whole Embryo Culture – Emily W. Y. Tung, Louise M. Winn
14. Rat Whole Embryo Culture – Craig Harris
15. Rabbit Whole Embryo Culture – Terence R. S. Ozolinš
16. Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology – Brendon K. Warner, Jonathan K. Alder, Arminda Suli
17. Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish – Jonathon T. Hill
18. Assessment of Histiotrophic Nutrition Using Fluorescent Probes – Craig Harris
19. Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization – Jeffery R. Barrow
20. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos – Robert M. Zucker, John M. Rogers
21. Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection – Danielle M. Drake, Aaron M. Shapiro, Peter G. Wells
22. Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA – Shama Bhatia, Peter G. Wells
23. Western Analysis of Breast Cancer 1 Protein (BRCA1) – Danielle M. Drake, Peter G. Wells
24. Regulation and Control of AP-1 Binding Activity in Embryotoxicity – Terence R. S. Ozolinš
25. Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) – Shama Bhatia, Jason Matthews, Peter G. Wells
26. The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity – Rebecca D. Maciver, Terence R. S. Ozolinš
27. Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining – Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, Michelle Graf

Editors Biography

Jason M. Hansen

Department of Physiology and Developmental Biology, Brigham Young University, Provo, USA

Louise M. Winn

Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada