Tietz Textbook of Clinical Chemistry and Molecular Diagnostics

Description

Select, perform, and evaluate the results of new and established laboratory tests. Now fully searchable, this classic reference features extended content for clinical chemists, pathologists, and laboratory managers. It offers encyclopedic coverage of the field that defines analytical criteria for the medical usefulness of laboratory procedures, introduces new approaches for establishing reference ranges, describes variables that affect tests and results, and more.

Key Features

• NEW! Internationally recognized chapter authors are considered among the best in their field.
• UPDATED! Expanded molecular diagnostics section with 12 chapters that focus on emerging issues and techniques in the rapidly evolving and important field of molecular diagnostics and genetics ensures this text is on the cutting edge and of the most value.
• NEW! Comprehensive list of reference intervals for children and adults with graphic displays developed using contemporary instrumentation.
• NEW! Standard and international units of measure make this text appropriate for any user, anywhere in the world.
• NEW! 22 new chapters that focus on applications of mass spectrometry, hematology, transfusion medicine, microbiology, biobanking, biomarker utility in the pharmaceutical industry, and more!
• NEW! Expert Editor, Nader Rifai, and Senior Editors, Andrea Rita Horvath and Carl T. Wittwer, bring fresh perspectives and help ensure that the most current information is presented.
• UPDATED! Thoroughly revised and peer-reviewed chapters provide you with the most current information

Table of Contents

1. SECTION I Basics of Laboratory Medicine
   1. Clinical Chemistry and Molecular Diagnostics
   2. Statistical Methodologies in Laboratory Medicine: Analytical and Clinical Evaluation of Laboratory Tests
   3. Quality Management in the Medical Laboratory*
   4. Specimen Collection and Processing
   5. Preanalytical Variation and Pre examination Processes
   6. Quality Control of the Analytical Examination Process
   7. Biological Variation
   8. Establishment and Use of Reference Intervals
   9. Evidence-Based Laboratory Medicine*
   10. Biobanking*
   11. Laboratory Support of Pharmaceutical, In Vitro Diagnostics, and Epidemiologic Studies*
2. SECTION II Analytical Techniques and Applications
   1. Principles of Basic Techniques and Laboratory Safety*
   2. Optical Techniques
   3. Electrochemistry and Chemical Sensors
   4. Electrophoresis
   5. Chromatography
   6. Mass Spectrometry
   7. Sample Preparation for Mass Spectrometry Applications*
   8. Mass Spectrometry Applications in Infectious Disease and Pathogens Identification*
   9. Development and Validation of Small Molecule Analytes by Liquid Chromatography-Tandem Mass
   10. Spectrometry*
   11. Proteomics*
   12. Enzyme and Rate Analysis
   13. Immunochemical Techniques
   14. Microfabrication and Microfluidics and Their Application in Clinical Diagnostics*
   15. Cytometry*
   16. Automation in the Clinical Laboratory*
   17. Point-of-Care Testing*
3. SECTION III Analytes
   1. Amino Acids, Peptides, and Proteins
   2. Serum Enzymes
   3. Enzymes of the Red Blood Cell*
   4. Tumor Markers
   5. Kidney Function Tests
   6. Carbohydrates
   7. Lipids, Lipoproteins, Apolipoproteins, and Other Cardiovascular Risk Factors
   8. Electrolytes and Blood Gases
   9. Hormones
   10. Vitamins and Trace Elements
   11. Hemoglobin, Iron, Bilirubin
   12. Porphyrins and the Porphyrias
   13. Therapeutic Drugs and Their Management
   14. Clinical Toxicology
   15. Toxic Elements
   16. Body Fluids*
4. SECTION IV Molecular Diagnostics
   1. Principles of Molecular Biology
   2. Genomes and Variants
   3. Nucleic Acid Isolation*
   4. Nucleic Acid Techniques
   5. Molecular Microbiology
   6. Genetics, 1025
   7. Solid Tumor Genomics
   8. Genetic Aspects of Hematopoietic Malignancies
   9. Circulating Tumor Cells and Circulating Tumor DNA
   10. Circulating Nucleic Acids for Prenatal Diagnostics
   11. Pharmacogenetics*
   12. Identity Testing*
5. SECTION V Pathophysiology
   1. Nutrition: Laboratory and Clinical Aspects*
   2. Diabetes Mellitus
   3. Cardiac Function
   4. Kidney Disease
   5. Disorders of Water, Electrolytes, and Acid–Base Metabolism
   6. Liver Disease
   7. Gastric, Pancreatic, and Intestinal Function
   8. Monoamine-Producing Tumors*
   9. Bone and Mineral Metabolism
   10. Pituitary Function and Pathophysiology
   11. Adrenal Cortex
   12. Thyroid Disorders
   13. Reproductive Endocrinology and Related Disorders
   14. Pregnancy and Its Disorders
   15. Newborn Screening and Inborn Errors of Metabolism
6. SECTION VI Hematology and Coagulation 
   1. Hemostasis*
   2. Red Blood Cell Morphology and Indices With Clinical Chemistry Interface*
   3. Automated Hematology*
7. SECTION VII Microbiology
   1. Bacteriology*
   2. Antimicrobial Susceptibility Testing*
   3. Mycobacteriology*
   4. Mycology*
   5. Parasitology*
   6. Virology*
8. SECTION VIII Transfusion Medicine
   1. Blood Groups, Pretransfusion Testing, and Red Blood Cell Transfusion*
   2. Blood Components, Blood Donor Screening, and Transfusion Reactions*
9. Appendix
10. Reference
11. Information for the Clinical Laboratory

Authors Biography

Nader Rifai

Affiliations and Expertise
Professor of Pathology, Harvard Medical School Louis Joseph Gay-Lussac Chair in Laboratory Medicine Director of Clinical Chemistry, Boston Children’s Hospital, Boston, MA USA

A. Rita Horvath

Andrea Rita Horvath’s key research interests include evidence-based laboratory medicine (EBLM) including evidence-based monitoring, guideline development, and evaluation of new biomarkers and overdiagnosis. She holds advisory roles on test utilization and reimbursement policy at the National Prescribing Service of Australia and Medicare. She has published 160 research papers and 16 book chapters and has been an invited speaker at over 100 international conferences.

Affiliations and Expertise
Professor, School of Medical Sciences, University of New South Wales, Sydney, Australia

Carl T. Wittwer

Dr. Wittwer is currently the medical director of Immunologic Flow Cytometry at Associated Regional and University Pathologists (ARUP), Salt Lake City, UT. In the early 1990s, he initiated molecular diagnostics at ARUP by forming its first molecular lab. From 2002 to 2012 he served as medical director of the Advanced Technology Group at ARUP. He is board certified in anatomic and clinical pathology by the American Board of Pathology.

Affiliations and Expertise
Professor of Pathology, University of Utah School of Medicine, Salt Lake City, UT USA